FIG. 3.

ceacam1a splice variant expression in murine tissues, primary cells, and cell lines. (A) Schematic representing ceacam1a splice variants. (B to G) RNAs isolated from 4-week-old C57BL/6 mouse tissues (B and E), primary cell cultures (C and F), and cell lines (D and G) were analyzed by qRT-PCR for ceacam1a expression of 2 versus 4 Ig-like domains (B to D) or short versus long cytoplasmic tails (E to G). Results are expressed as numbers of cDNA copies of ceacam1a per million cDNA copies of actb. Error bars represent standard errors of the means (n = 3). *, P ≤ 0.05; **, P ≤ 0.005 (determined by paired t tests). Data are representative of two independent experiments. 2D, two extracellular Ig-like domains; 4D, four extracellular Ig-like domains; S, short cytoplasmic tail due to exclusion of exon 7; L, long cytoplasmic tail due to inclusion of exon 7.