TABLE 1.
Primer sequences for qRT-PCR
| Target | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product size (bp) | GenBank accession no. |
|---|---|---|---|---|
| Mouse targets | ||||
| Beta-actin gene | CAGATGTGGATCAGCAAGCAGGA | CGCAGCTCAGTAACAGTCCGCCTA | 90 | NM_007393 |
| ceacam1a | ||||
| Total | GCCTCAGCACATCTCCACAAAG | CCTCAATGGTGACTTCAGCAGTG | 109 | NM_001039185 |
| 2D | CCTGGCGCTTGGAGCCTTTG | GCTGAGTCACTGGCTGGTGT | 263 | NM_001039187 |
| 4D | GGCCTCAGTAGGACCACAGTCAAGA | CGACAAGCAGGTCAGGGTCACAG | 120 | NM_001039185 |
| S | GCATCGTGATTGGAGTTGTG | GTTGTCAGAAGGAGCCAGATCC | 99 | NM_001039187 |
| L | GGGAAGTGACCAGCGAGAT | GTGGTTGGAGGCTGAGGGTTTGT | 52 | NM_001039185 |
| ceacam2 | CATCTCCACAAAGGGCAGGTTCC | TGTGCCAGCTAAAGGCCGAGAC | 187 | NM_007543 |
| psg16 | GCTTCGAGGGCTTTCCTGGTACA | TGTCGGGCAATCTCAAGGTTCCTA | 63 | NM_007676, U34272 |
| MHV target | ||||
| mRNA7 (nucleocapsid) | GGCGTCCGTACGTACCC | GGTCAGCCCAAGTGGTC | 167 | X00990 |