FIG. 3.

Effects of apoE deletion mutations on the apoE-NS5A interaction, as determined by co-IP. Huh-7.5 cells in 10-cm cell culture dishes were transfected with an apoE-specific siRNA or an NSC siRNA at 50 nM. In the meantime, wild-type apoE3 and individual deletion mutants were ectopically expressed by transfecting cDNA vectors into the HCV-infected cells. After 48 h, cell lysates were subjected to immunoprecipitation using a monoclonal anti-apoE antibody (mAb23) or an isotype-matched normal mouse IgG as a negative control. NS5A in the precipitate was detected by Western blotting using an NS5A monoclonal antibody.