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. 2010 Aug 25;84(21):11470–11478. doi: 10.1128/JVI.01338-10

FIG. 2.

FIG. 2.

(A) Schematic diagram of putative transcriptional control elements of the Ad5 UXP promoter by TRANSFAC analysis. Triangles represent CCAAT boxes; octagons represent E2F sites. Numbers indicate the location of each element relative to the transcription initiation site (+1). Details of nucleotide changes at each site are shown underneath. WT, wild type; Mut, mutant. (B) Effects of putative cis-element mutations on UXP transcription. (C) Quantification of Ad DNA template. A549 cells were infected in triplicate with wild-type Ad5 or the indicated promoter mutants at 10 PFU/cell. At 20 h p.i., total RNA and nuclear DNA were isolated and analyzed by Q-RT-PCR and Q-PCR, respectively. Values are normalized to that for Ad5. Data represent means ± standard deviations (SD) of results from triplicate cultures assayed in triplicate. (C) The P values of results for Cp, CC, or CE versus Ad5 are 0.095, 0.272, and 0.068, respectively. Abbreviations correspond to mutations at the following sites: Cp-proximal I-CCAAT box; Cd-distal I-CCAAT box; CC-double I-CCAAT boxes; EE-double E2F mutations (sites at bp −123 and −387); CE-double I-CCAAT box plus double E2F site mutations (sites at bp −123 and −387).