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. 2010 Aug 25;84(21):11470–11478. doi: 10.1128/JVI.01338-10

FIG. 5.

FIG. 5.

Transient-transfection analysis of promoter activity at 5′-end-flanking regions of UXP. (A) Schematic representation of the 5′-end-flanking region relative to the transcription initiation site; (B) luciferase assay on A549 cells; (C) luciferase assay on HEK293 cells. (B, C) Reporter plasmids containing the firefly luciferase cDNA linked with 5′-end-flanking regions of UXP were cotransfected with a control plasmid expressing Renilla luciferase. Transfection was performed in triplicate, and individual samples were analyzed using the dual-luciferase kit. Promoter activity is presented as the ratio of fLuc to rLuc (fLuc/rLuc). Data are means ± SD of results from two independent experiments.