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. 2010 Aug 18;84(21):11245–11254. doi: 10.1128/JVI.00372-10

FIG. 5.

FIG. 5.

SPICE and EMICE are also expressed on the cell surface in the presence of A56. SPICE is expressed on cells cotransfected with vaccinia virus A56wt. (A) Scatter plot of SPICE cotransfected with A56wt. (B) Histogram of SPICE expression on A56-positive cells. The black line is SPICE transfected with vaccinia virus A56wt, and the shaded gray area is SPICE transfected with A56mut3. (C) Scatter plot of EMICE transfected with VACV A56. (D) Histogram of CCP expression on virus-infected cells. RK-13 cells were infected with ECTV, VACV, or vv-VCPko and then stained with a polyclonal anti-VCP antibody. Shown is a histogram of surface staining due to EMICE (black line), VACV (dotted line), or vv-VCPko (gray shaded area). (E) Scatter plot of EMICE cotransfected with ECTV A56. (F) Histogram of EMICE expression of cells cotransfected with ECTV A56 (black line), VACV A56 (dotted line), or VACV A56mut3 (gray shaded area).