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. 2010 Aug 11;84(21):11164–11174. doi: 10.1128/JVI.01278-10

FIG. 6.

FIG. 6.

Avian NS1 sequesters Scribble in insoluble complexes. Cultures of 293T or A549 cells were infected with the indicated influenza viruses at an MOI of ∼2, and whole-cell extracts were prepared at 8 h postinfection. Cell lysates were fractionated into soluble and insoluble fractions. Amounts of soluble (S) and insoluble (I) cell fractions that represented equal numbers of cells were loaded on an 8% SDS-polyacrylamide gel. The indicated proteins were examined by immunoblotting.