FIG. 6.
Avian NS1 sequesters Scribble in insoluble complexes. Cultures of 293T or A549 cells were infected with the indicated influenza viruses at an MOI of ∼2, and whole-cell extracts were prepared at 8 h postinfection. Cell lysates were fractionated into soluble and insoluble fractions. Amounts of soluble (S) and insoluble (I) cell fractions that represented equal numbers of cells were loaded on an 8% SDS-polyacrylamide gel. The indicated proteins were examined by immunoblotting.