FIG. 8.

S protein colocalization with WT and mutant M proteins. 293T cells were transfected with pCAGGS vectors containing WT or mutant M proteins and the S gene. Cells were fixed at 12 h after transfection and analyzed by immunofluorescence using mouse and goat antibodies against the M and S proteins, respectively. Alexa Fluor 488-conjugated mouse and Alexa Fluor 594-conjugated goat secondary antibodies were used to visualize the localizations of the M and S proteins, respectively. Singly expressed M and S proteins are shown in the top two panels. Colocalizations of the M and S proteins are shown in the merged images on the right.