FIG. 1.

HCMV increases BiP protein and mRNA levels. (A) Western analysis probing for BiP accumulation during a HCMV infection time course; this reiterates previously reported results (5). (B) cDNA was generated from total RNA harvested from mock-infected (M) or HCMV-infected HFs at the indicated times postinfection (hpi) and subjected to quantitative PCR analysis using primers specific for BiP and GAPDH. BiP mRNA was normalized to GAPDH mRNA. (C) RNA was harvested from untreated (−) or thapsigargin (Thaps)-treated (+) uninfected HFs and analyzed as described in panel A. Fold activation in panels A and B is relative to the level in the mock sample, which was set to 1.