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. 2010 Aug 25;84(21):11479–11486. doi: 10.1128/JVI.01330-10

FIG. 4.

FIG. 4.

The HCMV MIE proteins (MIEPs) activate the BiP promoter. (A) A vector control plasmid or plasmids expressing the genomic MIE region or the individual MIE proteins IEP72 and IEP86 were coelectroporated into HFs with WT-BiP-Luc or mut-BiP-Luc and harvested 48 h postelectroporation for luciferase analysis. Fold activation was determined by normalizing luciferase levels to those of the vector control, set at 1. The Oct-TATA promoter control is described in the text. It cannot be activated by the MIE proteins, but it remains activatable by the herpes simplex virus VP16 transactivator (17). (B) HFs stably expressing luciferase under the control of the WT BiP promoter were transfected with plasmids expressing IEP72, IEP86, and GFP. Protein lysates were harvested 48 h postelectroporation for luciferase and Western analysis. Actin was used as a loading control for the Western analysis.