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Particles treated with 0.2% NP-40 are noninfectious and display a decrease in diameter. Detergent-treated and untreated particles were subjected to ultracentrifugation in a continuous 10 to 50% sucrose gradient. (A) Detergent treatment eliminated infectivity and resulted in a substantial loss of HCV RNA. (B) Particle diameter decreased from ∼60 to ∼45 nm with detergent treatment. Inset shows representative negatively stained particles. Scale bar, 50 nm.
