TABLE 2.
Comparison of quantum (maximal) yields of IFN and relative efficiency of IFN induction
| rgTK/OR/71 designationa | No. of aa residues of NS1 protein (1-X. . .Y)b | QY of IFN (U/1.0 × 107 cells) | No. of IFPs inducing the QY of IFN | IFN yield (U/106 PFP) | Relative IFN induction efficiencyc |
|---|---|---|---|---|---|
| Wild type | 1-230 | 4,000 | 1.0 × 107d | 400 | 1.0 |
| D-del pc2 (vac−)e | 1-115. . .125 | 14,000 | 1.0 × 107d | 1,400 | 3.5 |
| D-del pc1 (vac−)e | 1-80. . .90 | 17,000 | 1.0 × 107d | 1,700 | 4.3 |
| D-del pc4 (vac+)e | 1-91. . .93 | 42,500 | 5.4 × 106f | 8,000 | 20.0 |
| D-del pc3 (vac+)e | 1-69. . .86 | 72,000 | 5.4 × 106f | 13,300 | 33.3 |
See footnote a in Table 1.
See footnote b in Table 1.
Calculated relative to the IFN U/106 IFP value for the wild-type virus.
The lowest number of IFPs required to infect all (i.e., 1.0 × 107) cells in order to induce a QY of IFN for type r ≥ 1 dose-response curves.
Arranged in the order of increasing effectiveness as LAIV on the basis of the titer of antibodies against heterologous virus that they induced (44).
The number of IFPs infecting 27% (i.e., 2.7 × 106) of the cells in order to induce a QY of IFN for type r = 2 dose-response curves. Each of these cells is infected with 2 IFPs. The total number of IFPs contributing to the QY equals 5.4 × 106. See Materials and Methods for calculations.