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. 2010 Sep 1;84(21):11440–11447. doi: 10.1128/JVI.01030-10

FIG. 4.

FIG. 4.

Pancaspase inhibitor (Z-Asp-CH2-DCB) assays in 293T cells at 24 h posttransfection. (A) Effects of various concentrations of Z-Asp-CH2-DCB on the percentage of cell death induced by eGFP-PCV1ORF3. Error bars represent 95% confidence interval for the standard error of the mean from three independent experiments (n = 3). Values that are significantly different (P < 0.05) are indicated by an asterisk. MeOH, methanol; ddH2O, double-distilled water. (B) Effects of various concentrations of Z-Asp-CH2-DCB (0, 10, and 100 μM) on the percentage of cells with hypodiploid DNA content. (C) Effects of various concentrations of Z-Asp-CH2-DCB (0, 10, and 100 μM) on eGFP-PCV1ORF3-induced pyknosis (leftmost columns for both eGFP-PCV1ORF3 and eGFP-PCV2ORF3) and eGFP-PCV1ORF3-induced PS externalization (rightmost columns for both eGFP-PCV1ORF3 and eGFP-PCV2ORF3). For eGFP-PCV1ORF3-induced pyknosis (the leftmost columns for both eGFP-PCV1ORF3 and eGFP-PCV2ORF3), electronic volume (EV) is shown on the y axis, and side scatter (SS) is shown on the x axis. The arrow indicates the percentage of pyknosis in transfected cells. For eGFP-PCV1ORF3-induced PS externalization (rightmost columns for both eGFP-PCV1ORF3 and eGFP-PCV2ORF3), 7-AAD is shown on the y axis, and AV-PE is shown on the x axis. The bottom right quadrant shows the percentage of PS externalization in transfected cells. +ve, positive.