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. 2010 Aug 18;84(21):11134–11144. doi: 10.1128/JVI.01293-10

FIG. 2.

FIG. 2.

LANA is critical for KSHV to reduce IL-4-induced expression of the B-cell activation marker. (A) BJAB cells infected with GFP-KSHV (BJAB-K) and uninfected BJAB cells, (B) primary B cells (CD19+) gated from human PBMCs with or without KSHV-GFP (Bac36) at 7 days postinfection, and (C) lentivirus-mediated LANA knockdown (shLANA) or JSC and BC3 scramble control (shCtrl) cells were individually cultured in the presence or absence of IL-4 for 2 h. The cells were fixed with and stained with anti-CD40-APC or anti-Ki-67-PE. The average ratios of each of the stained positive cells are shown on the histograms. The efficiency of KSHV infection and LANA knockdown is presented in the right panels as the percentage of GFP fluorescence detected by flow cytometry.