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. 2010 Aug 25;84(21):11461–11469. doi: 10.1128/JVI.00538-10

FIG. 3.

FIG. 3.

Knockdown of NFX1-91 decreased p105 transcription. (A) p105 mRNA stability assay. HCT116 cells stably expressing shN91 number 1 or shScr cells were treated with actinomycin D, and total RNA was extracted at the indicated time points and detected by qPCR. The RNA ratio was normalized to an internal control, 36B4. (B) Knockdown of NFX1-91 decreased p105 promoter activity. HCT116 cells with knockdown of NFX1-91 were transiently cotransfected with a luciferase reporter driven by the p105 promoter and a β-Gal reporter. Luciferase activity was significantly reduced in NFX1-91 knockdown cells compared to the control cells (*, P < 0.02). These data are representative of two independent experiments, each of which was conducted in triplicate. The error bars indicate standard errors.