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. 2010 Aug 18;84(21):11056–11066. doi: 10.1128/JVI.00008-10

FIG. 1.

FIG. 1.

Knockdown of REGγ reduces coxsackieviral progeny titers. HeLa cells were transiently transfected with the REGγ siRNA (30 nM) or a scramble control siRNA for 24 h, followed by mock infection or CVB3 infection (MOI = 1) for various times as indicated (A) or for 16 h (B, C, D). (A) Supernatants of infected cells were harvested, and progeny virion titers were measured by a plaque assay. The results are presented as means ± SD (n = 3). *, P < 0.01 for comparison to the scramble siRNA control. Similar results were observed with HEK293 cells. (B) Western blot analysis was performed to detect caspase-3, p21, REGγ, and β-actin (loading control). (C) Caspase-3 activities were measured using a synthetic fluorogenic substrate, and the results are expressed as means ± SD (n = 3). *, P < 0.01 for comparison to the siRNA control. (D) HeLa cells were transfected with REGγ or control siRNAs for 24 h and then infected with CVB3 for 16 h in the presence of zVAD (50 μM). Plaque assay results are shown as means ± SD (n = 3). *, P < 0.01 for comparison to the scramble siRNA control.