FIG. 2.

Overexpression of REGγ promotes CVB3 replication. HEK293 tet-inducible REGγ cells were grown in the presence or absence of doxycycline (Dox) for 24 h, followed by mock or CVB3 infection (MOI = 1) for various times as indicated. (A) Cell lysates were collected, and expression of viral capsid protein VP1, REGγ, and β-actin (loading control) was detected by Western blot analysis. (B) Supernatants of infected cells were harvested, and progeny virion titers were measured by a plaque assay. The results are presented as means ± SD (n = 3). #, P < 0.001 for comparison to control cells without Dox induction.