FIG. 3.

Overexpression of REGγ decreases p21 and p53 levels. HeLa cells (A) or HEK293 cells (B) were transiently transfected with Flag-tagged REGγ expression vector or control plasmid pcDNA for 48 h. Cell lysates were collected, and expression of p53, p21, REGγ, and β-actin (loading control) was detected by Western blot analysis. Protein expression was quantitated by densitometric analysis using National Institutes of Health ImageJ 1.41o and normalized to the level for the control vector-transfected cells, which was arbitrarily set to a value of 1.0. Relative protein levels are listed below each panel. −, empty-vector control.