FIG. 4.

Blockage of p21 and p53 degradation by proteasome inhibitor lactacystin is associated with decreased virus protein expression. (A) HeLa cells were mock infected or infected with CVB3 (MOI = 10) for different time courses as indicated. Western blot analysis was carried out for detection of p53, p21, VP1, and β-actin. (B) HeLa cells were infected with CVB3 (MOI = 10) in the presence of increasing concentrations of lactacystin as indicated. Cell lysates were collected at 7 h postinfection for Western blot analysis of p21, p53, VP1, and β-actin.