FIG. 6.

Overexpression of p53 attenuates the effect of REGγ on promotion of CVB3 replication. HEK293 tet-inducible REGγ cells were grown in the presence or absence of doxycycline (Dox) as indicated for 24 h, followed by transient transfection with either p53 (+) or empty vector (−). Twenty-four hours later, cells were infected with CVB3 (MOI = 1) for 16 h. (Upper panel) Supernatants of infected cells were harvested, and progeny virion titers were measured by a plaque assay. The results are presented as means ± SD (n = 3). #, P < 0.001 for comparison to the vector control with induction of Dox (lane 2). (Lower panel) Cell lysates were collected for detection of viral capsid protein VP1, REGγ, p53, and β-actin (loading control) by Western blot analysis.