FIG. 2.

(a) Intracellular trafficking of pendrin variants in COS-7 cells. Merged representative images show green fluorescent protein–tagged wild-type or missense allele products (green) and concanavalin A staining (red) of plasma membrane. Colocalization (yellow) demonstrates targeting of pendrin to the plasma membrane. Scale bars = 20 μm. (b) Time course of ³⁶Cl⁻ efflux from representative oocytes and (c) rate constants (mean ± SE) for each group of (n) oocytes expressing wild-type or variant pendrin in extracellular medium containing 96 mM Cl⁻, 0 Cl⁻/5 mM I⁻, or 0 Cl⁻/0 I⁻, with gluconate as equimolar substituent anion. (d) Time course of ³⁶Cl⁻ efflux from representative oocytes and (e) rate constants (mean ± SE) for each group of (n) oocytes expressing wild-type or variant pendrin in extracellular medium containing 96 mM Cl⁻, 72 mM Cl⁻/24 mM HCO₃⁻, or 0 Cl⁻/24 mM HCO₃⁻/72 mM gluconate. Color images available online at www.liebertonline.com/gtmb.