Schematic diagrams illustrating (a) nonhierarchical sequencing, (b) hierarchical integrated sequencing-DHPLC, and (c) nonhierarchical DHPLC simulated screens of a composite cohort of 247 East Asian EVA subjects from this and six other studies (Tsukamoto et al., 2003; Park et al., 2005; Wu et al., 2005b; Hu et al., 2007; Wang et al., 2007; Lee et al., 2008). Numbers of subjects are in circles. Asterisks indicate numbers of DHPLC analysis temperatures.