Skip to main content
. Author manuscript; available in PMC: 2010 Oct 12.
Published in final edited form as: Traffic. 2008 Aug 6;9(10):1618–1628. doi: 10.1111/j.1600-0854.2008.00799.x

Figure 3. Arabidopsis AtVMA21a and AtVMA21b are stably expressed in yeast cells.

Figure 3

Immunoblot analysis of membrane fractions from vma2Δ yeast cells transformed with N-terminal HA-tagged constructs of Vma21p (pLG277), AtVMA21a (pLG270) or AtVMA21b (pLG266). 20 ug of membrane protein sample was loaded in each lane of a SDS-PAGE gel, resolved by electrophoresis, transferred to nitrocellulose and probed with antibodies recognizing the HA epitope, Vph1p and Dpm1p.