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. Author manuscript; available in PMC: 2010 Oct 12.
Published in final edited form as: Traffic. 2008 Aug 6;9(10):1618–1628. doi: 10.1111/j.1600-0854.2008.00799.x

Figure 4. Arabidopsis AtVMA21a and AtVMA21b demonstrate ER localization similar to the yeast V-ATPase assembly factor Vma21p.

Figure 4

Cell imaging of vma21Δ yeast cells transformed with N-terminal GFP-tagged constructs of ScVma21p (pLG278), AtVMA21a (pLG269) or AtVMA21b (pEB05). Prior to cell imaging samples were fixed (3.7% formaldehyde for 10 minutes) and incubated with 5 mg/ml Hoechst 33342 DNA binding dye. The panels on the right show yeast cell morphology as viewed by differential interference contrast microscopy (DIC). The panels on the far left are a merge of the green GFP fluorescence (second set of panels from the left) with the magenta-stained nucleic acid fluorescence (third set of panels from the left).