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. Author manuscript; available in PMC: 2010 Oct 12.
Published in final edited form as: Transplantation. 1993 Jun;55(6):1272–1277. doi: 10.1097/00007890-199306000-00012

FIGURE 3.

FIGURE 3

LD51: Chimerism demonstrated with PCR. After PCR amplification of HLA-DRB genes with “generic” oligo primers and hybridization of this material with allele-specific oligo probes, the recipient tissues were typed as DR4 (probe 1004) and DR7 (probe 1006). The donor blood was positive for probes 1004 (DR4), shared with the recipient, and 7011 (DR2). The amplified DNAs from donor blood (DB), recipient lymph nodes (RL), and recipient skin (RS) obtained using DR2 “specific” oligo primers were successfully hybridized with the allele-specific oligo probes 7011 and 8603, which recognize the DR2 allele of the donor. Probes 7002, 7003, and 8601 were negative. C is the negative control. In this experiment, the blood of the recipient (RB) was not positive.