Fig. 1.

A → I RNA editing of pri–miR-376 RNAs. (A) Hairpin structures of six human pri–miR-376 RNAs are shown. Editing sites (red A's) are numbered with the 5′ end of the human miR-376a1-5p sequence counted as +1. Regions processed into mature miRNAs are highlighted in green. The two most highly edited A's (+4 and +44 sites, red As) are highlighted in black. The genomically encoded G at +4 is also highlighted in black. The genomic distance between miRNA genes is indicated by the numbers at the bottom. (B) Analysis of pri–miR-376a1 and pri–miR-376b RNAs in human and mouse brains (wild-type and ADAR2^−/−) and pri–miR-376c RNAs in mouse E11.5 embryos (wild-type and ADAR1^−/−) by sequencing of reverse transcription polymerase chain reaction (RT-PCR) products. Thus, an A → I RNA editing site is detected as an A → G change in the cDNA sequencing chromatogram. Editing of human pri–miR-376b at the +44 site is almost 100%, as seen by the presence of a sole G peak without an A peak. Editing sites are indicated by red arrows.