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. Author manuscript; available in PMC: 2010 Oct 12.
Published in final edited form as: Science. 2007 Feb 23;315(5815):1137–1140. doi: 10.1126/science.1138050

Fig. 3.

Fig. 3

Physiological effects of miR-376a RNA editing. (A) Confirmation of the edited miR-376a targets. Relative luciferase activities in HeLa cells cotransfected with unedited miR-376a-5p (black bars) and edited miR-376a-5p (A → G at the +4 site) (blue bars), respectively. Reciprocal experiments were also conducted; for example, miR-376a-5p edited-version target sites challenged by unedited miR-376a and vice versa. (B) Luciferase activities were examined in HeLa cells cotransfected with a negative control cel-miR-67 (gray bars) and edited miR-376a-5p (A → I at the +4 site) (red bars), respectively. [(A) and (B)] The luciferase activities were compared statistically by Mann-Whitney U tests. Significant differences are indicated by asterisks, P < 0.05. Error bars, SEM (n = 3, where n is the number of independent measurements taken). (C) Western blot analysis of TTK and PRPS1 expression levels in the wild-type and ADAR2−/− mouse cortex and liver. Significant differences are indicated by asterisks, P < 0.05. Error bars, SEM (n = 3). (D) Uric-acid levels in the wild-type and ADAR2−/− mouse cortex and liver. Significant difference is indicated by an asterisk, P < 0.01. Error bars, SEM (n = 6).