Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Oct 4;191(1):45–60. doi: 10.1083/jcb.201003034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Ismail et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — BMI1 and RING2 are recruited to IRIF. (A) U2OS cells were irradiated with 2 Gy, extracted with 0.5% Triton X-100, and stained with antibodies recognizing BMI1 (green in composite) and γ-H2AX (red in composite). Maximum intensity projections are shown for each image. The top row is without deconvolution; the middle row is after deconvolution of the original fluorescence z series. The arrows show examples of PcG bodies. (B) Indirect IF of RING2 after irradiation with 2 Gy IR. The top left panel is a maximum intensity projection image of RING2 (green in bottom panels), the middle panel shows the same image after enhancement of gamma, and the right panel shows γ-H2AX (red in bottom panels). BMI1 and RING2 localize to sites of DSBs in addition to the PcG bodies, which remain highly enriched in PcG proteins after IR treatment. Arrows point to RING2 foci (PcG bodies) that do not colocalize with γ-H2AX foci. Bars, 5 µm.