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. 2010 Oct 4;191(1):45–60. doi: 10.1083/jcb.201003034

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© 2010 Ismail et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Dynamics of BMI1 and RING2 in living cells. U2OS cells expressing GFP-BMI1 or GFP-RING2 were monitored after microirradiation using time-lapse microscopy. (A) GFP-BMI1 and GFP-RING2 accumulation at laser track sites was quantified. The integrated intensity in the microirradiated areas was determined (n = 15) and the percentage of maximum value was plotted versus time. (B) The recruitment of GFP-tagged DSB repair proteins NBS1, MRE11, RNF8 was quantified for comparison. Error bars represent standard error from two independent experiments.