Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Oct 4;191(1):45–60. doi: 10.1083/jcb.201003034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Ismail et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — The RING domain of BMI1 is required for its accumulation at DNA damage sites. (A) Schematic representation of BMI1 deletion constructs used in B. Amino acid numbers are indicated. (B) BMI1 KO MEFs were transfected with WT BMI1 or the individual myc-tagged BMI1 deletion constructs shown in A. Immunoblots of extracts prepared from these cells were probed with myc antibody. As a loading control, the membrane was probed for actin. (C) BMI1 KO cells reconstituted with different myc-tagged BMI1 deletion constructs were microirradiated and double-stained with the myc (top) and PAR (bottom) antibodies. Bar, 5 µm.