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. 2010 Oct 4;191(1):129–139. doi: 10.1083/jcb.201005026

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© 2010 Mesnard and Constam

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Figure 3. — CLIP imaging reveals the presence of redundant Furin and Pace4 activities in the ICM of murine blastocysts. (A) Box plot of cell surface CFP/YFP ratios of E3.5 CLIP embryos from Furin and Pace4 single or double heterozygous intercrosses. CLIPm transgenic embryos are shown for comparison. The dot indicates a CFP/YFP ratio >0.4. (B) Blastocysts from Furin^+/−;Pace4^+/− intercrosses without (top row) or with (middle row) CFP at the surface of ICM cells. A CLIPm transgenic is shown for comparison (bottom row). (C) Heat maps of CFP/YFP ratios in the ICM of the blastocysts in A. (B and C) Error bars are means ± SD. Bar, 50 µm.