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. 2010 Oct 4;191(1):129–139. doi: 10.1083/jcb.201005026

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© 2010 Mesnard and Constam

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Figure 4. — Zygotic Furin;Pace4 DKO mutants show alternative PC activity at E5.5. (A) Whole mount in situ hybridization analysis of Nodal mRNA in embryos from Furin^+/−;Pace4^+/− intercrosses at stage E5.5–5.75. (B) Heat maps of CFP/YFP ratios in DKO and wild-type CLIP embryos and in CLIPm controls at E5.5. (C) From top to bottom, analysis of CFP and YFP signals in E6.5 wild type and DKO expressing CLIP, CLIPm, and nontransgenic embryos. Insets show the mean relative intensities of YFP (yellow) and CFP (blue) at the surface of epiblast cells. Dashed outlines indicate the boundary between the epiblast (ep) and visceral endoderm (ve). Bars, 50 µm. (D) CFP/YFP ratios were measured at the cell surface of E6.5 CLIP embryos from Furin^+/−;Pace4^+/− intercrosses or E6.5 CLIPm embryos. The fold difference was calculated relative to the mean CFP/YFP ratio of wild-type embryos. The asterisk indicates a significant difference as determined by t test (P < 0.05). Error bars are means ± SD.