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. 2010 Oct 4;191(1):61–74. doi: 10.1083/jcb.200912046

Figure 5.

Figure 5.

Effect of Sds22 depletion on substrate phosphorylation and centromere binding of aurora B. (A–C) Representative images and quantification of metaphase cells fixed and stained with anti–phospho-Ser92 MCAK and total MCAK (A), anti–phospho-Hec1 and total Hec1 (B), and anti-phospho–CENP-A and total CENP-A (C) antibodies in fixed HeLa cells 48 h after transfection with control (con) or Sds22-specific duplexes (see Materials and methods). Intensities were normalized relative to general kinetochore markers (Hec1 and ACA as indicated). Box plots and statistical analysis are as in Fig. 3 (E and F). Bars, 10 µm.