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. 2010 Oct 4;191(1):115–128. doi: 10.1083/jcb.201004118

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© 2010 Fridolfsson and Starr

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Figure 5. — Nuclear migration in kinesin-1 and dynein-regulatory mutant hyp7 precursors. (A and B) DIC images from a representative time-lapse sequence of klc-2(km11) (A) and nud-2(ok949);bicd-1(RNAi) (B) nuclear migration events. t = 0 min when the tip of cell 12 reached the opposite seam cell boundary. Anterior is to the left. (A’ and B’) Cell borders are outlined in black, nuclei migrating left to right are purple, and nuclei migrating right to left are green. Hyp7 cells are numbered 9–16, beginning with the posterior pointer cells. Bar, 10 µm. (C) Quantification of side by side nuclear alignment. Ratios represent the number of nuclei that could be scored out of total nuclei. Times are shown in minutes. Some nuclei failed to reach the dorsal midline before filming stopped at least 40 min after intercalation (*), and others could not be seen (°). The final column shows the percentage of nuclei that failed to complete migration.