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. 2010 Oct 4;191(1):141–154. doi: 10.1083/jcb.201007026

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© 2010 Mick et al.

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Figure 2. — coa3Δ yeast cells are respiratory deficient and lack cytochrome oxidase. (A) Wild-type (WT), coa3Δ, and cox14Δ yeast cells were spotted in serial 10-fold dilutions on fermentable (glucose) and nonfermentable (glycerol) media. (B) Enzyme assays of isolated mitochondria. Means of four independent experiments (SEM; n = 4). (C) Solubilized mitochondria were separated by BN-PAGE and analyzed by Western blotting. (D) Mitochondria (Mito) were separated by SDS-PAGE and analyzed by Western blotting.

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