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. 2010 Oct 4;191(1):141–154. doi: 10.1083/jcb.201007026

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© 2010 Mick et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Mss51 complexes are dynamic in nature. (A) In organello labeling of mitochondrial translation products was performed in the indicated strains, expressing Mss51^HA. Coimmunoprecipitation experiments were analyzed as in Fig. 3 E. Autoradiograph signals were quantified using ImageQuant TL software. The ratios of signals for Cox1, Cox2, and Cob between eluate and total were calculated and normalized to the Cox1 eluate/total ratio in wild type (WT; 100%). Means of three independent experiments (SEM; n = 3) are shown. (B) Mss51^HA and wild-type mitochondria were solubilized and analyzed by BN-PAGE and Western blotting using anti-HA antibodies. (C) Mitochondria isolated from Mss51^HA yeast cells treated with chloramphenicol (CAP) or cycloheximide (CHX) were analyzed as in B. Brackets indicate the molecular mass range of the Mss51 complexes. Lanes 1–4 in the bottom panels are cropped from the top panels and displayed with increased contrast for Cox1. (D) Solubilized mitochondria from pretreated cells were subjected to coimmunoprecipitation with anti-HA and control antibodies and analyzed by SDS-PAGE and Western blotting.

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