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. 2010 Oct 4;191(1):141–154. doi: 10.1083/jcb.201007026

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© 2010 Mick et al.

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Figure 7. — Lack of Coa1 stalls Mss51 in a 250-kD complex. (A) Mss51^HA mitochondria were solubilized, and samples were split and incubated with indicated antibodies. Antibodies with bound material were precipitated with protein A–Sepharose, and unbound material was analyzed by BN-PAGE and Western blotting using anti-HA antibodies. Respiratory chain supercomplexes and carrier translocase (TIM22) were detected with anti-Cox1 or anti-Tim54 antibodies, respectively. (B) Mitochondria from the indicated mutants expressing Mss51^HA were analyzed as in A. (C) Antibody depletion experiments were performed in coa1Δ mitochondria as in A. For comparison, untreated wild-type (WT) mitochondria were analyzed in lane 1.

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