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. 2010 Oct 4;191(1):199–210. doi: 10.1083/jcb.201006116

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© 2010 Libersou et al.

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Figure 8. — The incubation of G_th with liposomes at low pH induces the formation of tubular structures. (A) At pH 6, G_th, inserted into liposomes that were initially spherical, forms a local network (indicated by the arrow and enlarged in the top right frame), favoring the formation of tubular structures. (B) At pH 5.5, G_th forms more extensive, regular arrays at the surface of liposomes, resembling those formed by G at the surface of the virus. Spherical vesicles are nevertheless still visible (arrow). (C) At pH 5.2, only rigid tubular protein–lipid structures are observed, at the surface of which G_th displays quasi-helical symmetry. All the samples were negatively stained for electron microscopy observation.