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. Author manuscript; available in PMC: 2010 Oct 12.
Published in final edited form as: RNA Biol. 2008 Oct 9;5(4):263–272. doi: 10.4161/rna.7165

Figure 7.

Figure 7

Wobble positions G5761 and G5929 within the loop E motif are critical for the inhibition of RNase L. RNase L activity in reactions containing 20 nM RNase L, 20 nM 2–5A, 100 nM FRET probe and 100 nM of the indicated viral RNAs. (A) poliovirus ciRNA (blue line), poliovirus ciRNA G5761A (purple line), poliovirus ciRNA G5761U (brown line), poliovirus ciRNA G5761C (green line). (B) poliovirus ciRNA (blue line), poliovirus ciRNA G5929U (purple line), poliovirus ciRNA G5929C (brown line), poliovirus ciRNA G5929A (green line). A negative control reaction incubated without RNase L (probe alone, grey line). A positive control reaction containing FRET probe, RNase L, and 2–5A without poliovirus ciRNA (probe + RNase L + 2–5A, black line). A control reaction containing 100 nM of poliovirus ORF (2123, yellow line, poliovirus nucleotides 5889–6181). RFU plotted versus time of incubation.