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. 2010 Oct 12;5(10):e13262. doi: 10.1371/journal.pone.0013262

Figure 2. Validation of genes differentially regulated by TNF-α by QRT-PCR.

Figure 2

C2C12 myotubes were treated with 10 ng/ml of TNF for 18h followed by isolation of total RNA and QRT-PCR. Untreated myotubes under similar conditions were taken as control. The relative expression values (normalized with β-actin) from the QRT-PCR analysis were plotted for each gene are mean ± SD (n = 3). ‘*’ represents the statistical significance (p-value ≤0.01). A). Data presented here show that mRNA levels of NFKB1, NFKB2, IκBα, IL-6, VCAM1, Ccl5, Cxcl5, and Ccl2 were significantly increased in TNF-treated C2C12 cells. B). Data presented here show that mRNA levels of MyoD, Notch1, and Timp-2 were significantly reduced in TNF-treated C2C12 myotubes.