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. 2010 Oct 12;5(10):e13267. doi: 10.1371/journal.pone.0013267

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A/B. CD4CD25⁻ and CD4CD25^hi cells were isolated with Dako MoFlow. DNA was extracted and demethylation (%) of FOXP3 was measured with real-time PCR. C. Intracellular FOXP3 protein was measured in CD4CD25^hi cells as described in methods. N = 5, mean ± SEM were shown in B, one representative sample for A/C.