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. 2010 Oct 12;5(10):e13267. doi: 10.1371/journal.pone.0013267

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Demethylation (%) of FOXP3 in whole cord blood was measured with real-time PCR. FOXP3 mRNA expression was measured with real-time RT-PCR in unstimulated (A) and LpA-stimulated CBMCs (B), which were shown as delta ct to 18S. Lower delta ct represents higher mRNA expression. N = 48; correlation was analyzed with Pearsons correlation coefficient.