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. 2010 Oct 12;5(10):e13315. doi: 10.1371/journal.pone.0013315

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Gao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Positive stain seen with nitroblue tetrazolium/5-bromo-4-choloro-3-indolyl phosphate (purple-blue, Roch). Slides counterstained with nucleic fast red solution. (A) Positive stain (with NP sense probe) in nuclear and cytoplasm of pneumocytes (arrows). (B) Positive stain (with NP antisense probe) in nuclei and cytoplasm of Endotheliocyte from aortopulmonary (arrows). (C) Positive stain (with NP antisense probe) in lymphocytes of oxter lymph-node (arrows). (D) Positive stain (with NP antisense probe) in lymphocytes and monocytes from spleen (arrows). (E) Positive stain (with NP antisense probe) in nuclei and cytoplasm of neuron from Cerebrum cortex (arrow). (F) Positive stain (with NP antisense probe) in cytoplasm of epithelial cells from Colon (arrow). (G) Positive stain (with NP antisense probe) in lymphocytes of liver (arrow). (H) Positive stain (with HA antisense probe) in cytoplasm of endotheliocyte of renal contex (arrow). (I) Positive stain (with NP antisense probe) in nuclei of epithelial cells of ureter. Original magnifications: (A) ×20, (B–I) ×40.