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. 2010 Oct 12;8(10):e1000503. doi: 10.1371/journal.pbio.1000503

Figure 10. Rhythmic expression of nlp-36p::gfp is abolished in tax-2 mutants.

Figure 10

(A) Quantification of intestinal nlp-36p::gfp expression levels in wild-type (gray bars) or AFD-killed animals (red bars) at defined time points during entrainment (CW) followed by 3 d of free-running (WW) conditions. Error bars indicate the s.e.m. Data shown are from two independent experiments of 20 animals each. (B) Fluorescence intensities in wild-type animals (gray bars) or tax-2 mutant animals (red bars) carrying the nlp-36p::gfp transgene entrained to temperature cycles (CW/WW). Error bars indicate the s.e.m. Data shown are from two independent experiments. (C) Cycling of selected light-entrained transcripts in wild-type animals (gray) and in tax-2(p671) mutants (red) quantified via qRT-PCR. The probabilities of circadian cycling (pF 24) by appending each independent qPCR time course experiment are indicated. (D) Cycling of selected light-entrained transcripts in wild-type animals (gray) and in lite-1(ce314) mutants (green) quantified via qRT-PCR. The probabilities of circadian cycling (pF 24) are indicated. The bars below the graphs in (C) and (D) denote the entrainment protocol, with white and black bars indicating the light and dark phases, respectively. Data shown are from two independent biological replicates per time point. Error bars indicate the s.e.m. n = 15–20 animals each.