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. 2010 Oct 12;8(10):e1000512. doi: 10.1371/journal.pbio.1000512

Figure 2. MBC-dependent microtubule depolymerization overcomes the spindle checkpoint block.

Figure 2

(A) Mad2GFP localization in cdc11-119 cells blocked at 36.5°C for 4 h in the presence of DMSO (left) or 50 µg/ml MBC (right). (B) Facs profiles of cdc11-119 cut7-446 double mutant cells at 25°C (cycling) and at 36.5°C in the presence of either DMSO (−) or 50 µg/ml MBC (+). Cells were grown in EMM and nitrogen starved for 3 h prior to shift up as previously described [31]. Notably control DMSO-treated cdc11-119 cut7-446 cells show accumulation of cut cells, despite the presence of the cdc11-119 mutation. (C) Facs profile of cdc11-119 nuf2-2 cells at 25°C (cycling) and at 36.5°C in the presence of either DMSO (−) or 50 µg/ml MBC (+).