Figure 7. Inhibition of cAMP signaling abolishes stochastic rhythms from Bmal1 ^−/− SCN explants.

(A–C) Representative records of PER2::LUC rhythms of the SCN explants from WT (blue) and Bmal1 ^−/− (red) mice. Data are shown following a medium change (day 8); shaded area indicates when SCN explants were chronically treated with vehicle solution (A), 1 µM MDL (B), or 10 µM H-89 (C). WT SCN explants recovered rhythms immediately following the washout of MDL (B, left) or H-89 (C, left); however, almost all Bmal1 ^−/− explants required second washout to reinitiate their rhythms (B and C; right).