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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Cell Death Differ. 2010 Aug 13;17(11):1672–1683. doi: 10.1038/cdd.2010.97

Figure 6. Bmf isoforms localize preferentially to the outer mitochondrial membrane.

Figure 6

(A) NIH3T3 cells were transiently transfected with HA-tagged versions of BmfS or BmfCUG. 24h after transfection cells were fixed and prepared for immunofluorescence. (B) NIH3T3 cells were left untreated or cultured in the presence of 2μM cytochalasin D for 16h. Cells were harvested, washed in PBS and lysed prior subcellular fractionation and alkaline stripping of mitochondria. Pellet and supernatant (SN) fractions were separated by SDS-PAGE, transferred onto nitrocellulose membranes and subjected to immunoblotting using anti-Bmf mAb (17A9). Membranes were reprobed with antibodies specific for the mitochondrial transmembrane protein CoxIV or cytoplasmic GAPDH.