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. Author manuscript; available in PMC: 2010 Oct 12.
Published in final edited form as: Cancer Cell. 2006 Nov;10(5):389–399. doi: 10.1016/j.ccr.2006.08.027

Figure 1.

Figure 1

Many putative BH3 mimetics do not kill like BH3-only proteins

A: The viability of wild-type MEFs (WT) or Bax- and Bak-deficient MEFs (DKO) 24 h after infection with the indicated retroviruses. Expression of the cDNA encoding the BH3-only protein BimS or tBid was linked by an IRES to that of GFP, and the viability of GFP+ve cells determined by PI exclusion.

B: Representative wells showing colony formation by wild-type (WT) or Bax/Bak-deficient (DKO) MEFs after infection with the control parental retrovirus or one expressing BimL.

C–H: The viability (% cells excluding PI) of WT or Bax- and Bak-deficient (DKO) MEFs treated for 24 h with graded doses of the indicated putative BH3 mimetics.

I: Colonies formed by wild-type (WT) or Bax/Bak-deficient (DKO) MEFs in the presence of no treatment, HA14-1 or Antimycin A.

J: The relative affinities (IC50 in nM) of a BimBH3 peptide (as previously reported; Chen et al., 2005) and several putative BH3 mimetic compounds for Bcl-2 and/or Bcl-w. The affinities were measured in solution competition assays (Chen et al., 2005).

Data in A and C–H represent means ± SD from 3 independent experiments.