Figure 6.
Polarized SFG spectra collected in the Amide I region from magainin 2 at the PS surface at concentrations of a) 200 nM, b) 400 nM, and c) 800 nM. d) Time-dependent ppp SFG signal at 3070 cm−1 collected from the deuterated PS/water interfaces; peptide was added at t=100 seconds to reach a concentration of 200 nM. The laser was blocked at t=70 sec to demonstrate that signal was present. Polarized SFG spectra collected in the C-H/O-H stretching frequency region from magainin 2 at concentrations of e) 400 nM, and f) 800 nM.