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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: J Neurosci Res. 2010 Nov 15;88(15):3295–3307. doi: 10.1002/jnr.22484

Fig 4. MAPK/ERK signaling is required for IGF-1-enhancement of oligodendrocyte differentiation on laminin.

Fig 4

(A) Oligodendrocytes were differentiated on laminin for 3 days in the presence or absence of MEK inhibitor (PD98059) or vehicle control, as well as in the presence or absence of 50 ng/ml IGF-1. Following treatment, oligodendrocyte lysates were evaluated by Western blot to determine MBP and CNP protein relative to actin loading control. A representative western blot from 4 independent experiments is shown. (B) Densitometry to determine levels of MBP protein following IGF-1 treatment in vehicle or PD98059-treated oligodendrocytes. Bars represent the mean (± sem) change in MBP protein relative to actin from 4 independent experiments (*p<0.05). PD98059 abolishes IGF-1-induced enhancement of MBP expression.